LOCUS pcDNA3.1(+) ZKSCAN1 MCS Exon Vector 6701 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression plasmid for expressing circular RNAs of a desired
sequence in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1(+) ZKSCAN1 MCS Exon Vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6701)
AUTHORS Kramer MC, Liang D, Tatomer DC, Gold B, March ZM, Cherry S, Wilusz
JE
TITLE Combinatorial control of Drosophila circular RNA expression by
intronic repeats, hnRNPs, and SR proteins.
JOURNAL Genes Dev. 2015 Oct 8.
PUBMED 26450910
REFERENCE 2 (bases 1 to 6701)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6701
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2295..2312)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 2301..2525
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2571..2999
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2658..2677)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2868..2889
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3008..3028)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3013..3342
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3193..3328
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3255..3274
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3409..4203
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3463..3482)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4073..4092
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 4377..4498
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4414..4433)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4468..4487
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4547..4563)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4547..4563)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4560..4582)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4571..4587
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4595..4625)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4640..4661
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4778..4795)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4949..5534)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5029..5048)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5705..6565)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6328..6347
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6566..6670)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcagt
961 gacagtggag attgtacagt tttttcctcg atttgtcagg attttttttt tttgacggag
1021 tttaacttct tgtctcccag gtaggaagtg cagtggcgta atctcggctc actacaacct
1081 ccacctcctg ggttcaagcg tttctcctgc ctcagctttc cgagtagctg ggattacagg
1141 cgcctgccac catgccctgc tgacttttgt atttttagta gagacggggt ttcaccatgt
1201 tggccaggct ggtcttgaac tcctgaccgc aggcgattgg cctgcctcgg cctcccaaag
1261 tgctgagatt acaggcgtga gccaccaccc ccggcctcag gagcgttctg atagtgcctc
1321 gatgtgctgc ctcctataaa gtgttagcag cacaaatcac tttttgtaaa ggtacgtact
1381 aatgactttt tttttatact tcaggatatc atcgataccg gtttaattaa cacgtgggta
1441 accgttaacc cgcggaggta agaagcaagg tttcatttag gggaagggaa atgattcagg
1501 acgagagtct ttgtgctgct gagtgcctgt gatgaagaag catgttagtc ctgggcaacg
1561 tagcgagacc ccatctctac aaaaaataga aaaattagcc aggtatagtg gcgcacacct
1621 gtgattccag ctacgcagga ggctgaggtg ggaggattgc ttgagcccag gaggttgagg
1681 ctgcagtgag ctggaatcat gccactactc caacctgggc aacacagcaa ggaccctgtc
1741 tcaaaagcta cttacagaaa agaattaggc tcggcacggt agctcacacc tgtaatccca
1801 gcactttggg aggctgaggc gggcagatca cttgaggtca ggagtttgag accagcctgg
1861 ccaacatggt gaaaccttgt ctctactaaa aatatgaaaa ttagccaggc atggtggcac
1921 attcctgtaa tcccagctac tcgggaggct gaggcaggag aatcacttga acccaggagg
1981 tggaggttgc agtaagccga gatcgtacca ctgtgctcta gccttggtga cagagcgaga
2041 ctgtcttaaa aaaaaaaaaa aaaaaagaat taattaaaaa tttaaaaaaa aatgaaaaaa
2101 agctgcatgc ttgttttttg tttttagtta ttctacattg ttgtcattat taccaaatat
2161 tggggaaaat acaacttaca gaccaatctc aggagttaaa tgttactacg aaggcaaatg
2221 aactatgcgt aatgaacctg gtaggcatta gcggccgctc gagtctagag ggcccgttta
2281 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
2341 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
2401 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
2461 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
2521 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
2581 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
2641 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
2701 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
2761 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
2821 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
2881 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
2941 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
3001 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
3061 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
3121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
3181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3241 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
3301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
3361 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
3421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
3481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
3541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
3601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
3661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
3721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
3781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
3841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
3901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
3961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
4021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
4081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
4141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
4201 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
4261 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
4321 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
4381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
4441 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
4501 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
4561 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
4621 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
4681 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
4741 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
4801 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
4861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
4921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
4981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
5041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
5101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
5161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
5221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
5281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
5341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
5401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
5461 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
5521 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
5581 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
5641 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
5701 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
5761 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
5821 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
5881 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
5941 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
6001 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
6061 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
6121 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
6181 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
6241 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
6301 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
6361 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
6421 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
6481 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
6541 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
6601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
6661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
 ZKSCAN1 MCS Exon Vector载体图谱.png)
